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Part 01: Intro to Cervid CWD Sample Submission Protocol
Michigan Department of Agriculture 2002 CWD
Coordinator, Dr Rachel Cezar and USDA Field VMO, Dr Joe Odziana compiled the following original presentation.
Updates are done on
an ongoing basis by Michigan Department of Agriculture personnel.
Include ALL cervidae over 12 months of age that are either
culled for slaughter at premise site or any type of off-site processing
facility. The program is utilizing a targeted, active, on-going surveillance to
reach 25% of your culled stock but no more than (30,35,40) head. When
considering culls to be tested INCLUDE the weak and/or thinnest animals
in the herd when available.
Also ANY animals euthanized or found dead due to
sickness or injury MUST be submitted for CWD testing. These will count toward
the total required number of head for the targeted surveillance for your herd.
There are 3 options for collection of samples for CWD
testing in cervids:
The first method is to remove and submit the entire
head(s) to the laboratory. Delivery is via the MDA courier service or if possible
deliver the head(s) directly to the MSU diagnostic lab or the MDA/USDA Atlanta Office.
The second and PREFERRED option is removal of the brain
stem through the foramen magnum (the opening where the spinal cord exits the
skull). Submit the brainstem along with
the MRLN's in 10% NB formalin supplied by MDA. Please use ground or air mail
delivery service when submitting your samples by means other then courier or
hand delivering to AHDL or MDA/USDA Atlanta Office.
The third method is to remove the entire brain then
separate out and submit the brainstem portion of the brain along with the
medial retropharyngeal lymph nodes (MRLN's) in 10% Neutral Buffered (NB)
formalin supplied by MDA. Please use ground or air mail delivery service when
submitting your samples by means other
then courier or hand delivering to AHDL or MDA/USDA Atlanta Office.
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