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Testing of Living Non-equine
Currently, testing of samples
from living non-equine species for evidence of WNV infection is limited. At the
DCPAH, two tests are available for canine serum and/or CSF.
capture ELISA can be run for dogs showing acute neurological signs. The
submittal form must be filled out
completely and must accompany the sample. The test requires approximately 2 ml
of serum, 5 ml of clotted whole blood or 1ml of CSF and takes 2 days to
complete. The Virology Section of DCPAH will perform this test on Tuesdays and
Thursdays and the results will be faxed. Samples have to be in the laboratory by
noon at the latest on the days the test is performed. The fee for this testing
capture ELISA is a screening test for previous exposure to WNV. The test
requires approximately 2ml of serum or 5 ml of clotted whole blood and takes 2
days to complete. Sera will be tested at a dilution of 1:100 and the results
will be reported as positive or negative. Positive results indicate that the dog
was previously exposed to field virus, but cannot be used to determine whether
the animal has protective immunity to WNV. The Virology Section of DCPAH will
perform this test on Thursdays and the results will be faxed. Samples have to be
in the laboratory by noon at the latest on the days the test is performed. The
fee for this testing is $25.00.
Neutralization (VN) Test, which can be run on serum from any species, will be
performed in the Immunodiagnostics Section of DCPAH. Two ml of serum or 5 ml of
whole blood are required. This test provides information on the titer of viral
neutralizing antibodies in serum. The fee for this test is $25.00.
Testing of Dead Non-equine
Necropsy of non-equine WNV
suspects will be similar to the protocol described under Laboratory Testing for
horses. Tissues will be examined microscopically, and if indicated, specific
tests for rabies virus and other arboviruses will be performed.